中文句式1

微生物生长动态采用BioSense oCelloScope高通量显微成像系统进行全程实时监测，设备依托透射成像扫描模式获取菌体覆盖面积时序变化信号，自动输出校正浊度TA值与视野覆盖率数据，全程恒温控制，无开盖扰动，实现长时间连续非破坏性观测微生物增殖、生物膜形成及菌体裂解全过程。

中文句式2

本研究使用丹麦BioSense公司生产的oCelloScope动态微生物成像分析仪采集生长曲线，仪器通过阵列式光学扫描对微孔板样品进行定时成像，软件同步记录时间-生物量时序数据，用于后续生长动力学拟合、AUC积分计算与表型特征解析。

英文句式1（最常用）

The time-course microbial growth was continuously monitored using an oCelloScope dynamic microbial imaging system (BioSense, Denmark). The device performs transmission scanning to record real-time coverage and corrected absorbance (TA) signals under constant temperature control, enabling non-invasive long-term observation of microbial proliferation, biofilm formation and cell lysis without plate opening.

英文句式2

An oCelloScope imager (BioSense, Denmark) was applied to acquire microbial growth curves in microplates. Time-resolved biomass data were exported for kinetic fitting, AUC calculation and phenotypic analysis of microbial strains.

二、抑菌实验专用句式（药物抑菌、噬菌体裂解、消毒剂药效方向）

中文

借助BioSense oCelloScope成像系统对微孔板内菌液进行间隔成像采集，完整记录受试抑菌物质作用下微生物生长延滞、生长速率下降、菌体裂解等动态变化，配套软件导出时序数据用于生长动力学参数求解与抑菌活性定量评价。

英文

Time-lapse imaging of bacterial suspensions in microplates was conducted on an oCelloScope system (BioSense, Denmark). Dynamic phenotypes including growth lag extension, growth rate reduction and cell lysis induced by antibacterial agents were recorded, and time-series data were exported for kinetic parameter calculation and quantitative antibacterial activity evaluation.

三、生物膜专用句式（生物膜形成、抗生物膜材料、清洗效果评价）

中文

采用BioSense oCelloScope显微成像系统原位观测微孔板底部生物膜附着量动态变化，依靠成像覆盖率量化生物膜累积水平，对比各组生物膜生长、成熟、剥离全过程差异。

英文

In-situ observation of biofilm accumulation at the bottom of microplates was achieved with the oCelloScope imaging platform (BioSense, Denmark). Biofilm biomass was quantified via imaging coverage to compare the whole developmental cycle of biofilm formation, maturation and detachment among treatment groups.

四、发酵动力学、菌株生长表型专用句式

中文

使用oCelloScope高通量成像分析仪测定菌株批次培养生长曲线，稳定的恒温培养环境减少温度波动带来的生长误差，高通量微孔布局可同步完成多菌株、多梯度条件平行筛选。

英文

Batch culture growth curves of strains were determined using the high-throughput oCelloScope analyzer (BioSense, Denmark). Stable thermostatic incubation minimized growth bias from temperature fluctuation, and the microplate layout supported parallel screening of multiple strains and concentration gradients simultaneously.

五、数据导出与后处理衔接句式（衔接Origin拟合、AUC计算、统计学分析）

中文

oCelloScope配套控制软件导出以培养时间、校正浊度TA、成像覆盖率为核心的CSV格式时序原始数据，将数据导入Origin软件开展梯形法AUC积分、生长动力学模型拟合（Logistic/Baranyi模型），完成定量数据统计分析。

英文

Raw time-series data containing incubation time, corrected absorbance (TA) and imaging coverage were exported as CSV files from the oCelloScope software. Subsequent trapezoidal AUC integration and growth kinetic model fitting (Logistic/Baranyi) were performed in Origin for quantitative statistical analysis.

六、精简短句（用于期刊方法段落篇幅受限场景）

中文精简版

微生物生长曲线由BioSense oCelloScope成像系统测定，恒温条件下连续成像记录菌体生物量动态变化。

英文精简版

Microbial growth curves were measured by an oCelloScope system (BioSense, Denmark) with continuous time-lapse imaging under constant temperature.

七、使用注意事项

1.仪器产地统一标注Denmark，厂商标注BioSense，仪器名称统一为oCelloScope，大小写保持全文一致；

2.核心指标TA（corrected absorbance）首次出现标注全称，后续直接简写；

3.区分使用场景，抑菌侧重菌体裂解、延滞期；生物膜侧重底部附着覆盖率；

4.不要与常规酶标仪OD值混写，明确表述为成像校正浊度/成像覆盖率，区别于传统光密度检测。