丹麦BioSense官方原始英文仪器说明书要点
一、说明书开篇定义与系统整体概述（Official Definition）
1. 产品官方全称
Automated Non-Invasive Time-Lapse Brightfield Imaging System for Microbial Growth & Morphology Analysis（微生物生长与形态自动化非侵入式明场延时成像系统），品牌归属：Danish BioSense ApS，配套软件：UniExplorer，整机可直接放置于标准恒温培养箱内长期连续运行。
2. 系统三大核心构成（手册强制标注）
1. Proprietary FluidScope™ patented optical scanning module（专利FluidScope光学扫描模组）；
2. High-precision XY motorized mechanical stage（高精度电动XY载台传动机构）；
3. UniExplorer desktop analysis software with licensed algorithms（带算法授权的UniExplorer分析软件）。
3. 官方适用样本范围
细菌、酵母菌、霉菌/丝状真菌、真菌孢子、混合菌群；拓展可检测哺乳动物细胞、药物结晶、花粉颗粒；仅适配液体悬浮培养体系，无需染色、固定、试剂标记，纯光学无损检测。
二、硬件部分说明书关键条款（Hardware Specification Manual）
1. FluidScope™专利光学核心硬性参数（原文重点）
1. Optical axis fixed tilt angle：6.25° relative to microplate horizontal plane（光轴相对微孔板水平面固定倾斜6.25°）；
2. Imaging mode：Z-stack vertical layered scanning，step size adjustable（Z轴堆栈逐层扫描，步长软件可调，常规推荐10μm）；
3. Camera resolution：5MP digital CCD，minimum detectable particle size 1.3μm，可识别单个真菌孢子、短芽管；
4. Illumination：low-power diffuse white LED transmitted light，no UV/laser radiation，zero phototoxicity to microbes（低功率漫射白光LED，无紫外/激光，无光毒性，不会惊扰菌体生长）。
2. 机械载台与通量限制（手册明确标注）
1. Single plate loading only：instrument can hold one microplate per run，no automatic plate stacker（单次仅装载1块微孔板，无自动换板机械手）；
2. Compatible plate formats：6/12/24/48/96-well flat-bottom transparent polystyrene microplates only；V-bottom、U-bottom、opaque plates prohibited（仅平底透明聚苯乙烯板，U/V底、不透明板禁止使用）；
3. Scanning speed：≤3 minutes for full 96-well plate with full Z-stack scan（96孔板全套Z轴扫描≤3分钟）；
4. Positioning repeat error：＜5μm，guarantee long-term time-lapse image registration alignment（重复定位误差＜5微米，保障十余天时序图像堆栈配准不偏移）。
3. 电气、环境与维保强制要求
1. Power：100–240V wide voltage DC low-consumption power supply，continuous operation heat generation extremely low；
2. Operating temperature range：20℃–40℃，designed for incubator internal placement；
3. Calibration cycle mandatory requirement：
- Daily user self-calibration：software background baseline correction before every experiment（每次实验前软件基线自校准）；
- Annual factory professional full optical calibration：once per year by authorized engineer，include tilt angle recheck，stage positioning calibration，pixel size metric calibration（每年一次厂家上门全光路深度校准）；
4. Forbidden operation：end-user shall not disassemble casing, adjust lens angle or wipe internal optical components（禁止用户拆机、调节镜头倾角、擦拭内部光学镜片）。
4. 出厂标配&附赠耗材（说明书附件清单原文）
1. Standard delivered items：main unit, power adapter, RJ45 network cable, locking plate holder, dust cover, UniExplorer permanent software license, operation manual PDF, one-year hardware warranty；
2. Complimentary trial consumables：5 pcs 96-well transparent plates, 1 glass slide adapter, cleaning wiper pack；
3. Consumables NOT included (need separate purchase)：mass 96-well plates, breathable sealing film, Mini Agar Disks, flow-cell microfluidic kit（大批量微孔板、透气封板膜、微型琼脂盘、微流控套件均为选购耗材）。
三、UniExplorer软件官方说明书核心规则（Software User Guide）
1. 语言设置官方标准步骤（原文操作路径）
1. Top menu bar：Tools → Options → Advanced tab；
2. Language dropdown select：Chinese (Simplified)；
3. Save setting, exit software via File→Exit（not close window directly）；
4. Restart program to activate Chinese interface；
5. Garbled text fix：modify Windows system non-Unicode locale to Chinese (Simplified, China)。
2. 三大底层授权算法（手册明确版权算法）
1. SESA algorithm (Segmentation and Extraction of Surface Area)：static impurity filtering，automatically eliminate medium crystal, drug precipitate, bubble, only retain microbial valid pixels（静态杂质过滤，长周期监测抗干扰核心）；
2. BCA algorithm (Background Corrected Absorption)：biomass area quantification，sensitivity 250× higher than standard OD microplate reader（生物量面积定量，灵敏度为传统OD法250倍）；
3. TA algorithm (Total Absorption)：equivalent OD value，for parallel comparison with turbidity method；
4. Supplementary morphological segmentation algorithm：20+ morphological parameters for bacteria, yeast and filamentous fungi（20余项形态学量化指标）。
3. 内置动力学拟合数学模型完整列表（Official Built-in Models）
1. Modified Gompertz Model（default recommended for fungi spore germination）；
2. Baranyi-Roberts Model（predictive microbiology gold standard for lag phase calculation）；
3. Logistic Model；
4. Richards Generalized Growth Model；
5. Exponential Growth Model；
6. Monod Substrate Limitation Model；
7. Linear Regression Model；
All models automatically calculate λ lag phase, μmax maximum growth rate, Xmax maximum biomass, R² goodness of fit。
4. 图像导出与数据导出官方权限说明
1. Raw image export permission (full licensed version only)：
- Single well single time-point best-focus original TIFF/JPG lossless pictures；
- Full Z-stack layered stack images per well；
- SESA binary segmentation annotated comparison images；
- Time-lapse MP4 video batch export；
File naming automatically contains experiment ID, well position, time point, stack layer for traceability；
2. Morphological parameter export：fully customizable checkbox selection，support two export dimensions：① single individual cell/hypha detailed data；② per-well average aggregated data，output format Excel/CSV；
3. Trial version limitation：only basic BCA biomass data available, no morphological parameter export and limited image export quantity。
5. 数据溯源与审计规范（英文手册合规条款）
All exported images, kinetic curves, parameter tables are one-to-one corresponding；complete experiment package can be packaged and migrated to another computer for re-analysis, meeting laboratory data retention and method validation requirements.
四、官方标注应用场景与性能优势（Application Note Key Points）
1. Antimicrobial susceptibility test (AST/MIC)：results available within 4 hours, 4 times faster than CLSI standard broth microdilution method (16–20h), adopted by US CDC for resistant bacteria detection；
2. Fungal spore germination, preservative inhibition efficacy, long-term dormancy spore resuscitation monitoring (up to 10+ days continuous run)；
3. Morphological phenotypic analysis：β-lactam induced bacterial filamentation, abnormal hyphae, spore swelling quantification；
4. Methodological verification：correlation validation between imaging area and CFU plate counting, establish alternative quantitative method；
5. Core advantage over OD reader：OD cannot distinguish biomass increase and morphological abnormality, this system realizes quantity quantification + visual phenotype double verification。
五、说明书明确风险警示与禁忌条款（Safety & Warning）
1. Do not frequently move the instrument after installation, vibration will cause optical axis offset and positioning deviation；
2. Long-period incubator internal operation: avoid condensate dripping on the top optical window；
3. Do not set ultra-high frequency continuous scanning, cumulative weak light will theoretically cause negligible slight stress to sensitive spores；standard 30min–4h interval scanning is completely safe；
4. The instrument cannot modify built-in algorithm formulas and add custom mathematical models, only select existing parameters for export.
本文内容根据 BioSense 官方英文说明书整理，软件版本、配置套餐存在差异，仪器交付配置、功能权限及售后条款均以正式采购合同与原厂装箱单为准，内容仅作技术参考，不具备验收与法律效力。
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